BPB Protamine Monoclonal Antibodies

$275.00 – $1,450.00

A new set of protamine monoclonal antibodies (MAbs) have been developed that recognize and bind to protamines P1 and/or P2. Similar to the Hup antibodies, these new MAbs recognize and bind to the isolated protamines and those in their native state (while bound to DNA in spermatids and sperm).

Description

A new set of protamine monoclonal antibodies (MAbs) have been developed that recognize and bind to protamines P1 and/or P2. Similar to the Hup MAbs, these new MAbs recognize and bind to the isolated protamines and those in their native state (while bound to DNA in spermatids and sperm). Reference: Balhorn, R., Steger, K, Bergmann, M, Schuppe, H.C., Neuhauser, S. and Balhorn, MC (2018) Systems Biology in Reproductive Medicine, 64(6): 424-447 PUBMED.

Our products are for use in laboratory research only.

While these new MAbs have only been tested for their selectivity in binding to human, mouse, stallion and bull protamines P1 and P2, the epitopes that have been identified indicate these antibodies should recognize protamines present in the spermatids and sperm of a much broader range of species than Hup1N, Hup2b or Hup1M.

The new antibodies have a number of features that differ from the Hup monoclonals. These include

1) a broader range of mouse antibody isotype,

2) the recognition of different protamine P1 and P2 epitopes,

3) applicability for use in chromatin immunoprecipitation assays, and

4) other uses that require the recognition of protamines P1 or P2 from most mammalian species and many marsupials, birds and reptiles.

Please see Useful Information Page for Protocols. Note: Protamines are very basic proteins and are insoluble in SDS, so please use acid-urea gels and not SDS gels.

Characteristics of the new BPB protamine monoclonal antibodies

ELISA titration curves showing P1/P2 antigen specificity by species: comparison of the new BPB MAbs with Hup MAbs

ELISA tritation curves showing the binding of the twelve new purified protamine monoclonal antibodies binding to human, mouse, stallion, and bull protamines

Additional information

Weight	0.05 lbs
Dimensions	0.05 × 0.05 × 0.05 in
MAb Type	BPB-2 A11, BPB-5 A2, BPB-6 F3, BPB-8 F5, BPB-9 F1, BPB-5 D1, BPB-11 C1, BPB-20 D4, BPB-26 B11, BPB-17 B8, BPB-26 F6, BPB-18 C10
Quantity (micrograms)	150ug ($275 US), 300ug ($450 US), 1,000ug ($1,450 US)

Protamine Epitopes

These synthetic peptides contain the protamine P1 and protamine P2 epitopes recognized by the antibodies Hup1N and Hup2B, respectively.

$100.00 – $400.00 Select options

HUP Protamine Monoclonal Antibodies

Hup1N, Hup2B and Hup1M anti-protamine monoclonal antibodies are provided as affinity purified reagents. Hup1N and Hup2B recognize and bind to protamine P1 and P2 molecules, respectively, from many types of mammals. Hup1M is selective for human protamine P1 only.

$275.00 – $1,450.00 Select options

Mammalian Protamines

Native isolated and synthetic forms of the mammalian protamine P1 and P2 proteins are available

Native isolated protamine molecules:
- Protamine P1 as isolated from sperm (bull, boar, ram)
- Protamine P1 isolated from sperm and then purified by HPLC (bull and hamster)
- Protamine P1P2 Mix as isolated from sperm (hamster, stallion, and human)
Synthetic HPLC purified human, stallion and mouse protamines

If you are interested in protamines isolated from the sperm of a species other than those listed here, please contact us – we may be able to help.

$100.00 – $1,700.00 Select options